Contributor submission NosL

mite_data/data/0e0c24a4-e4eb-11f0-abdb-8218ec668ab3.json

@@ -0,0 +1,69 @@
+{
+    "accession": "MITE9999999",
+    "status": "pending",
+    "changelog": [
+        {
+            "version": "1",
+            "date": "2025-12-29",
+            "contributors": [
+                "AAAAAAAAAAAAAAAAAAAAAAAA"
+            ],
+            "reviewers": [
+                "BBBBBBBBBBBBBBBBBBBBBBBB"
+            ],
+            "comment": "New entry."
+        }
+    ],
+    "enzyme": {
+        "name": "NosL",
+        "description": "Radical SAM enzyme",
+        "references": [
+            "doi:10.1021/cb900133x",
+            "doi:10.1038/nchembio.512"
+        ],
+        "databaseIds": {
+            "uniprot": "C6FX51",
+            "genpept": "ACR48341.1",
+            "mibig": "BGC0000610"
+        },
+        "cofactors": {
+            "organic": [
+                "SAM"
+            ],
+            "inorganic": [
+                "FeS"
+            ]
+        }
+    },
+    "reactions": [
+        {
+            "tailoring": [
+                "Carboxylation",
+                "Deamination",
+                "Decarboxylation"
+            ],
+            "description": "3-methyl-2-indolic acid (MIA) synthase involved in nosiheptide biosynthesis",
+            "reactionSMARTS": "[#7:1]\\[#6@:2](-[#6:13](-[#8:15])=[#8:14])-[#6:3]-[#6:4]1:[#6:8]2:[#6:9]:[#6:10]:[#6:11]:[#6:12]:[#6:7]:2:[#7:6]:[#6:5]:1>>[#6:3]-[#6:4]1:[#6:8]2:[#6:9]:[#6:10]:[#6:11]:[#6:12]:[#6:7]:2:[#7:6]:[#6:5]:1-[#6:13](-[#8:15])=[#8:14]",
+            "reactions": [
+                {
+                    "substrate": "N[C@@H](Cc1c[nH]c2ccccc12)C(=O)O",
+                    "products": [
+                        "Cc1c(C(=O)O)[nH]c2ccccc12"
+                    ],
+                    "isIntermediate": true,
+                    "description": "Removal of the Ca-N unit of L-tryptophan and shift of carboxylate to the indole ring."
+                }
+            ],
+            "evidence": {
+                "evidenceCode": [
+                    "In vitro assay",
+                    "Knock-out studies",
+                    "Site-directed mutagenesis"
+                ],
+                "references": [
+                    "doi:10.1038/nchembio.512"
+                ]
+            }
+        }
+    ]
+}